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If you are like me, you sometimes look at the fisheries Stocking Reports just to see what lakes are being stocked. When you get into these reports, you start seeing terms like “Pennask 2N” or “Fraser Valley AF3N” and wonder what the heck that means. The following is a quick overview of what all those stocking terms mean.

Strains of Trout:

The  Rainbow Trout in BC came from different locations as the glaciers receded during the last ice age. Trout from these  various locations evolved differently to adapt to their specific environmental conditions. These differences include feeding behavior, spawning behavior, size, appearance, habitat preference and environmental tolerances. Some of these are genetic based and are passed along to later generations of those fish. They are all Rainbow Trout but the genetic differences form different “strains” of Rainbow Trout. The last report I saw (2004), recognized eight different
strains in BC. These are:

• Pennask
• Blackwater
• Tzenzaicut
• Dragon
• Premier
• Tunkwa
• Gerrard
• Fraser Valley

As a note of caution however, I haven’t seen the Tunkwa strain listed in the Stocking Reports for some time. Three strains of Rainbows are usually found in the lakes of the Thompson-Nicola area around Kamloops. These are the Pennask, the Fraser Valley and the Blackwater. The Pennask strain is the most common Rainbow stocked in our area. The Fraser Valley strain is found in lakes like Edith, Jacko, Morgan, Six Mile and Red. The Blackwater strain is put into lakes such as Tunkwa, Stump, Logan, Lac des Roches, and Paul. A few small lakes near Kamloops on the Bonaparte Plateau have been stocked with the Dragon strain.

Specialty Treatments of Trout:

In addition to the “strain” of the trout, the stocking reports usually show designations regarding the type of fish being stocked. These are:

• AF All Female
• 2N Diploid
• 3N Triploid
• AF3N All Female Triploid

So what does all that mean? It refers to specialty treatments the trout have had or have not had to help with the fisheries management of our lakes.

All Female

For us anglers, there are advantages to having an all female population of trout in our favorite fishing lake. Females mature slower than males and thus go into the spawning cycle later in life providing us with longer angling opportunities. The loss of precocious males is eliminated and in an all female environment, the fish are less likely to go into the spawning phase.

The process to obtain all females is labor intensive for the fisheries folks. Female fry are exposed to testosterone derivatives that halt ovary development and instead, encourage the production testes. In essence, it produces a female with testes even though the Y male chromosomes are missing. Since these females lack a sperm duct, the milt

must be obtain by hand to mix with ordinary female eggs. Since no male Y chromosomes are involved, the newly hatched fish are all female.

Diploid

The term “ploidy” refers to the degree of replication of chromosomes. The chromosomes are linear sets of DNA molecules which form the basis of heredity. Most critters have two sets or strands of chromosomes. When reproduction occurs, cells divide equally and the chromosomes duplicate themselves in pairs. These are referred to as Diploid or 2N. These are the trout in an unaltered natural state. Both male and female offspring occur, mature, mate and can produce other diploid young. When you see the term diploid or 2N in the fisheries Socking Reports it means that no special treatments have occurred to enhance the growth of the fish. Because of cost considerations, these are
the most commonly stocked fish in our area.

Triploid

While the diploids (2N) have two strands of chromosomes, triploid (3N) fish have three strands of chromosomes. Since reproductive cell division occurs in pairs and triploids have three chromosome strands, the extra strand cannot be duplicated. This renders the triploid fish sterile and incapable of reproduction.

Triploids are created by applying heat or cold shocks, high pressure or certain chemical treatments to newly fertilized eggs. In BC, triploids are created by applying hydro static pressure or by the heated water treatment shortly after fertilization. What this does is prevent the second division of meiosis allowing an extra set of female chromosomes to be retained. The embryo will have one set of paternal chromosomes and two sets of maternal chromosomes.

All 3N females are sterile and won’t enter the spawning phase. Some males, however, will still undergo secondary sexual development, produce gonads and go into the spawning cycle even though they are sterile.

The advantages of triploids over diploids are:

• they live longer
• they have less mortality as a result of spawning
• they are less prone to disease
• they grow faster
• they grow larger since energy is transferred to growth rather than reproduction

Triploids can easily grow to 20 pounds and larger. In 2009, a 48 pound triploid was caught in Lake Diefenbaker, Saskatchewan. While this Rainbow takes the world record, there are arguments against allowing triploids into the record books.

……. Ron Newman

* Ron Newman has been fly fishing rainbow trout in our province for over 30 years, during which time he has accumulated an great amount of knowledge on the subject. Along with sharing some of that knowledge on sites like ours, and through the Kamloops Fly Fishers Association of which he is a member, Ron has also documented his fishing expertise in his book Rainbow Trout Fly Fishing: A Guide for Still Waters! We will be offering a review of this book in the near future … in the meantime please check it out here!

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Tags: 2N Diploid, 3N Triploid, AF All Female, AF3N All Female Triploid, BC Fish Stocking, Blackwater, Diploid, Dragon, Fraser Valley, Gerrard, Pennask, Premier, Ron Newman, Triploid, Trout Strains, Tunkwa, Tzenzaicut

This entry was posted on Friday, March 4th, 2011 at 7:00 am and is filed under Still Water Fly Fishing. You can follow any responses to this entry through the RSS 2.0 feed. You can leave a response, or trackback from your own site.